ارتباط بین پلی مورفیسم ۳۱۸C / T ژن CTLA ‐ ۴ و لوپوس اریتماتوز سیستمیک در بیماران ایرانی
Abstract
Background
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is an important negative regulator of T-cell response. It is a functional candidate gene connected with susceptibility to systemic lupus erythematosus (SLE). We analyzed the role of −۳۱۸C/T polymorphism in the promoter region of the CTLA-4 gene in Iranian patients suffering from SLE.
Methods
A total of 180 SLE patients and 304 healthy ethnically matched controls were enrolled in the study. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of these polymorphisms.
Results
The CC genotype was observed in 170 (94.5%) of the SLE patients, which was significantly different compared to the controls (251 [82.4%]; P = ۰٫۰۰۰۱, OR = ۳٫۵۱ ۹۵%CI = ۱٫۷۷–۷٫۵۳). T allele was significantly more common in the controls (9.2%) compared to SLE patients 2.8% (P = ۰٫۰۰۰۱, OR = ۰٫۲۶, ۹۵%CI = ۰٫۱۳–۰٫۵۳). There was no significant correlation between different genotypes and age, gender or family history of SLE in the studied population.
Conclusion
It can be concluded that −۳۱۸C/T polymorphism of CTLA-4 gene might play a significant role in the development of SLE in the Iranian patients.
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